Products
NFE2 antibody
- Product Name
- NFE2 antibody
- Catalogue No.
- FNab05692
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- nuclear factor(erythroid-derived 2), 45kDa
- UniProt ID
- Q16621
- Observed MW
- 45 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
K-562 cells were subjected to SDS PAGE followed by western blot with FNab05692(NFE2 antibody) at dilution of 1:500
IP Result of anti-NFE2 (IP:FNab05692, 4ug; Detection:FNab05692 1:500) with K-562 cells lysate 3440ug.
Immunohistochemistry of paraffin-embedded human colon cancer using FNab05692(NFE2 antibody) at dilution of 1:50
- Background
- Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2(HS2) of the beta-globin control region(LCR). This subunit(NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
