NDUFB8 antibody

Synonyms:ASHI antibody, CI ASHI antibody, Complex I ASHI antibody, NDUFB8 antibody
Catalogue No.:FNab05625Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IF, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
NDUFB8 antibody
Catalogue No.
FNab05625
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
NADH dehydrogenase(ubiquinone) 1 beta subcomplex, 8, 19kDa
Alternative Names
ASHI antibody, CI ASHI antibody, Complex I ASHI antibody, NDUFB8 antibody
UniProt ID
O95169
Observed MW
20 kDa
Application
Tested Applications
ELISA, WB, IHC, IF, IP
Recommended dilution
WB: 1:500-1:1000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
mouse liver tissue were subjected to SDS PAGE followed by western blot with FNab05625(NDUFB8 antibody) at dilution of 1:500
IP Result of anti-NDUFB8 (IP:FNab05625, 4ug; Detection:FNab05625 1:1000) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human liver using FNab05625(NDUFB8 antibody) at dilution of 1:50
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab05625 (NDUFB8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase(Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.