A2780 cells were subjected to SDS PAGE followed by western blot with FNab05599(NDC80 antibody) at dilution of 1:500
IP Result of anti-NDC80 (IP:FNab05599, 4ug; Detection:FNab05599 1:500) with A2780 cells lysate 960ug.
Immunohistochemistry of paraffin-embedded human brain using FNab05599(NDC80 antibody) at dilution of 1:100
Background
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity(PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore(PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules(PubMed:23085020).