Products
NDC80 antibody
- Product Name
- NDC80 antibody
- Catalogue No.
- FNab05599
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- NDC80 homolog, kinetochore complex component
- Alternative Names
- HEC antibody, HEC1 antibody, KNTC2 antibody
- UniProt ID
- O14777
- Observed MW
- 76 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
A2780 cells were subjected to SDS PAGE followed by western blot with FNab05599(NDC80 antibody) at dilution of 1:500
IP Result of anti-NDC80 (IP:FNab05599, 4ug; Detection:FNab05599 1:500) with A2780 cells lysate 960ug.
Immunohistochemistry of paraffin-embedded human brain using FNab05599(NDC80 antibody) at dilution of 1:100
- Background
- Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity(PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore(PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules(PubMed:23085020).
