HeLa cells were subjected to SDS PAGE followed by western blot with FNab05594(NCOR2 antibody) at dilution of 1:1000
Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using FNab05594 (NCOR2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L)
Background
Transcriptional corepressor. Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center(GC) reactions, mainly through the control of the GC B-cells proliferation and survival.