Products
NCKIPSD antibody
- Product Name
- NCKIPSD antibody
- Catalogue No.
- FNab05586
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- NCK interacting protein with SH3 domain
- Alternative Names
- AF3P21 antibody, SPIN90 antibody
- UniProt ID
- Q9NZQ3
- Observed MW
- 80 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab05586(NCKIPSD antibody) at dilution of 1:500
IP Result of anti-NCKIPSD (IP:FNab05586, 4ug; Detection:FNab05586 1:300) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab05586(NCKIPSD Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
- Background
- Has an important role in stress fiber formation induced by active diaphanous protein homolog 1(DRF1). Induces microspike formation, in vivo(By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42(By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.
