Products
NCK1 antibody
- Product Name
- NCK1 antibody
- Catalogue No.
- FNab05583
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- NCK adaptor protein 1
- Alternative Names
- NCK antibody
- UniProt ID
- P16333
- Observed MW
- 43kd
Application
- Tested Applications
- ELISA, WB, IHC, IF, FC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab05583(NCK1 antibody) at dilution of 1:400
IP Result of anti-NCK1 (IP:FNab05583, 4ug; Detection:FNab05583 1:500) with mouse brain tissue lysate 2640ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab05583(NCK1 antibody) at dilution of 1:100
- Background
- Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.
