Products
NANOG antibody
- Product Name
- NANOG antibody
- Catalogue No.
- FNab05541
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Nanog homeobox
- Alternative Names
- hNanog antibody, Homeobox protein NANOG antibody, NANOG antibody, Nanog homeobox antibody
- UniProt ID
- Q9H9S0
- Observed MW
- 39-48 kDa
Application
- Tested Applications
- ELISA, IF, WB
- Recommended dilution
- WB: 1:500-1:2000; IF: 1:20-1:200
Validated Images
Mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab05541( NANOG Antibody) at dilution of 1:1000
- Background
- Transcription regulator involved in inner cell mass and embryonic stem(ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating(ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
