Products
NAGA antibody
- Product Name
- NAGA antibody
- Catalogue No.
- FNab05537
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- N-acetylgalactosaminidase, alpha-
- Alternative Names
- Alpha galactosidase B antibody, D22S674 antibody, GALB antibody, NAGA antibody
- UniProt ID
- P17050
- Observed MW
- 48 kDa, 60-70 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab05537(NAGA antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human placenta using FNab05537(NAGA antibody) at dilution of 1:100
- Background
- NAGA belongs to the glycosyl hydrolase 27 family. It removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. It is required for the breakdown of glycolipids. Biosynthetic studies performed with cultured fibroblasts indicated that the human enzyme was synthesized as a 65kDa glycosylated precursor which was processed to a mature 48-kDa lysosomal form; both the precursor and mature forms had high mannose type oligosaccharide chains, but only the precursor’s mannose residues were phosphorylated. 90-117kd is a homodimer of NAGA.
