WB : 1:500-1:2000; IHC : 1:20-1:200; IF : 1:50-1:500
Validated Images
mouse kidney tissue were subjected to SDS PAGE followed by western blot with FNab05480(MYH9 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer using FNab05480(MYH9 antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells, using FNab05480(MYH9 antibody) at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Background
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation(in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10.