Products
MIF antibody
- Product Name
- MIF antibody
- Catalogue No.
- FNab05187
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- macrophage migration inhibitory factor(glycosylation-inhibiting factor)
- Alternative Names
- GIF antibody, GLIF antibody, L dopachrome isomerase antibody, L dopachrome tautomerase antibody, MIF antibody, MMIF antibody, Phenylpyruvate tautomerase antibody
- UniProt ID
- P14174
- Observed MW
- 12 kDa
Application
- Tested Applications
- ELISA, IHC, WB, IP, IF
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500
Validated Images
Y79 cells were subjected to SDS PAGE followed by western blot with FNab05187(MIF antibody) at dilution of 1:1000
IP Result of anti-MIF (IP:FNab05187, 4ug; Detection:FNab05187 1:1000) with mouse spleen tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab05187(MIF Antibody) at dilution of 1:200
Immunofluorescent analysis of ( -20°C Ethanol) fixed A549 cells using FNab05187 (MIF antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity(in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
