Products
MCM5 antibody
- Product Name
- MCM5 antibody
- Catalogue No.
- FNab05060
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- minichromosome maintenance complex component 5
- Alternative Names
- CDC46 antibody
- UniProt ID
- P33992
- Observed MW
- 82 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab05060(MCM5 antibody) at dilution of 1:500
IP Result of anti-MCM5 (IP:FNab05060, 4ug; Detection:FNab05060 1:500) with Jurkat cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human lymphoma using FNab05060(MCM5 antibody) at dilution of 1:100
- Background
- Acts as component of the MCM2-7 complex(MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity(By similarity). Interacts with MCMBP.
