Products
MCC antibody
- Product Name
- MCC antibody
- Catalogue No.
- FNab05046
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- mutated in colorectal cancers
- UniProt ID
- P23508
- Observed MW
- 98 kDa ; 113-118 kDa
Application
- Tested Applications
- ELISA, IHC, IP, WB, IF
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
human brain tissue were subjected to SDS PAGE followed by western blot with FNab05046(MCC antibody) at dilution of 1:500
IP Result of anti-MCC (IP:FNab05046, 3ug; Detection:FNab05046 1:500) with PC-3 cells lysate 5000ug.
Immunohistochemistry of paraffin-embedded human prostate cancer using FNab05046(MCC antibody) at dilution of 1:100
- Background
- Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase(PAK) activation(PubMed:18591935, PubMed:19555689, PubMed:22480440). Represses the beta-catenin pathway(canonical Wnt signaling pathway) in a CCAR2-dependent manner by sequestering CCAR2 to the cytoplasm, thereby impairing its ability to inhibit SIRT1 which is involved in the deacetylation and negative regulation of beta-catenin(CTNB1) transcriptional activity(PubMed:24824780).
