Products
MBD3 antibody
- Product Name
- MBD3 antibody
- Catalogue No.
- FNab05035
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- methyl-CpG binding domain protein 3
- UniProt ID
- O95983
- Observed MW
- 33 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
Y79 cells were subjected to SDS PAGE followed by western blot with FNab05035(MBD3 antibody) at dilution of 1:600
IP Result of anti-MBD3 (IP:FNab05035, 4ug; Detection:FNab05035 1:800) with mouse testis tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human endometrial cancer using FNab05035(MBD3 antibody) at dilution of 1:100
- Background
- Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself(PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides(in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides(PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
