Products

LIN28B antibody

Synonyms:CSDD2 antibody
Catalogue No.:FNab04785Reativity:Human, Mouse
Host:RabbitTested Application:ELISA, WB
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
LIN28B antibody
Catalogue No.
FNab04785
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
lin-28 homolog B(C. elegans)
Alternative Names
CSDD2 antibody
UniProt ID
Q6ZN17
Observed MW
27 kDa
Application
Tested Applications
ELISA, WB
Recommended dilution
WB: 1:500-1:2000
Validated Images
mouse embryo tissue were subjected to SDS PAGE followed by western blot with FNab04785(LIN28B antibody) at dilution of 1:300
Background
Suppressor of microRNA(miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts(pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA(PubMed:22118463). Does not act on pri-miR21(PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation(PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent(PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression(By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.