Products
LIG4 antibody
- Product Name
- LIG4 antibody
- Catalogue No.
- FNab04774
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ligase IV, DNA, ATP-dependent
- UniProt ID
- P49917
- Observed MW
- 100-104 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
mouse testis tissue were subjected to SDS PAGE followed by western blot with FNab04774(LIG4 antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human prostate cancer using FNab04774(LIG4 antibody) at dilution of 1:100
- Background
- Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining(NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
