Products
LEF1 antibody
- Product Name
- LEF1 antibody
- Catalogue No.
- FNab04745
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- lymphoid enhancer-binding factor 1
- UniProt ID
- Q9UJU2
- Observed MW
- 40-45 kDa
Application
- Tested Applications
- ELISA, IHC, WB, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
COLO 320 cells were subjected to SDS PAGE followed by western blot with FNab04745(LEF1 Antibody) at dilution of 1:300
IP Result of anti-LEF1 (IP:FNab04745, 4ug; Detection:FNab04745 1:300) with SW 1990 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human thymus using FNab04745(LEF1 antibody) at dilution of 1:100
- Background
- Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1(By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
