Products
ISCU antibody
- Product Name
- ISCU antibody
- Catalogue No.
- FNab04402
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- iron-sulfur cluster scaffold homolog(E. coli)
- Alternative Names
- NIFUN antibody
- UniProt ID
- Q9H1K1
- Observed MW
- 14-15 kDa, 18-19 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
mouse heart tissue were subjected to SDS PAGE followed by western blot with FNab04402(ISCU antibody) at dilution of 1:500
IP Result of anti-ISCU (IP:FNab04402, 3ug; Detection:FNab04402 1:500) with mouse heart tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human lung cancer using FNab04402(ISCU antibody) at dilution of 1:50
- Background
- Scaffold protein for the de novo synthesis of iron-sulfur(Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins(PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH(By similarity).
