Products
IFITM3 antibody
- Product Name
- IFITM3 antibody
- Catalogue No.
- FNab04144
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- interferon induced transmembrane protein 3
- Alternative Names
- 1 8U antibody, IFITM3 antibody, IP15 antibody
- UniProt ID
- Q01628
- Observed MW
- 14 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP, IF, FC
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:3000; IHC: 1:50-1:400; IF: 1:50-1:500
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab04144(IFITM3 Antibody) at dilution of 1:1000
IP Result of anti-IFITM3 (IP: FNab04144, 3ug; Detection: FNab04144 1:1500) with HeLa cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human colon cancer using FNab04144(IFITM3 antibody) at dilution of 1:100
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab04144 (IFITM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus(SARS-CoV), Marburg virus(MARV) and Ebola virus(EBOV), Dengue virus(DNV), West Nile virus(WNV), human immunodeficiency virus type 1(HIV-1) and vesicular stomatitis virus(VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase(v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state.
