Products
GST tag antibody
- Product Name
- GST tag antibody
- Catalogue No.
- FNab03684
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 2E9
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Recombinant protein
- Alternative Names
- Glutathione S-Transferase antibody, GST antibody, gst tag antibody
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:5000-1:50000; IP: 1:5000-1:20000
Validated Images
GST tagged recombinant protein(25ng/lane) was subjected to SDS-PAGE followed by western blot with anti-GST tag monoclonal antibody FNab03684 at dilution of 1:40000
IP result of anti-GST Tag(IP:FNab03684, 5ug; Detection:FNab03684 1:10000) with Total E. coli lysate (with pGEX4T fused plasmid) 1360 ug
- Background
- GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein.
