Products
GNB5 antibody
- Product Name
- GNB5 antibody
- Catalogue No.
- FNab03543
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- guanine nucleotide binding protein(G protein), beta 5
- UniProt ID
- O14775
- Observed MW
- 39-42 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab03543(GNB5 antibody) at dilution of 1:1000
IP Result of anti-GNB5 (IP:FNab03543, 4ug; Detection:FNab03543 1:500) with rat brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human ovary tumor using FNab03543(GNB5 antibody) at dilution of 1:100
- Background
- Heterotrimeric guanine nucleotide-binding proteins(G proteins) function as transducers downstream of G protein-coupled receptors(GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
