Products
GEMIN8 antibody
- Product Name
- GEMIN8 antibody
- Catalogue No.
- FNab03423
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- gem(nuclear organelle) associated protein 8
- Alternative Names
- FAM51A1 antibody
- UniProt ID
- Q9NWZ8
- Observed MW
- 32 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab03423(GEMIN8 antibody) at dilution of 1:400
IP Result of anti-GEMIN8 (IP:FNab03423, 3ug; Detection:FNab03423 1:400) with HepG2 cells lysate 1720ug.
Immunohistochemistry of paraffin-embedded human liver cancer using FNab03423(GEMIN8 antibody) at dilution of 1:50
- Background
- The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins(snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.
