Products
Flag tag antibody
- Product Name
- Flag tag antibody
- Catalogue No.
- FNab03155
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 2D10
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Flag tag
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000
Validated Images
Flagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03155(Flag tag antibody) at dilution of 1:5000
IP Result of anti-Flag tag (IP: FNab03155, 3ug; Detection: FNab03155 1:2000) with Recombinant protein protein lysate 500ug.
- Background
- Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This antibody is generated against 1xDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein.
