Products
ERp57/ERp60 antibody
- Product Name
- ERp57/ERp60 antibody
- Catalogue No.
- FNab02858
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- protein disulfide isomerase family A, member 3
- Alternative Names
- 58 kDa microsomal protein antibody, Disulfide isomerase ER 60 antibody, ER protein 57 antibody, ER protein 60 antibody, ER60 antibody, ERp57 antibody, ERp60 antibody, ERp61 antibody, GRP57 antibody, GRP58 antibody, HsT17083 antibody, P58 antibody, PDIA3 antibody, PI PLC antibody, Protein disulfide isomerase A3 antibody
- UniProt ID
- P30101
- Observed MW
- 57 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, FC, IP
- Recommended dilution
- WB: 1:500-1:2400; IP: 1:1000-1:4000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
L02 cells were subjected to SDS PAGE followed by western blot with FNab02858(PDIA3 antibody) at dilution of 1:1000
IP Result of anti-ERp57/ERp60 (IP: FNab02858, 4ug; Detection: FNab02858 1:2000) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human lung cancer using FNab02858(PDIA3 antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells, using PDIA3 antibody FNab02858 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Background
- PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
