Products
EIF5B antibody
- Product Name
- EIF5B antibody
- Catalogue No.
- FNab02730
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- eukaryotic translation initiation factor 5B
- Alternative Names
- IF2 antibody, KIAA0741 antibody
- UniProt ID
- O60841
- Observed MW
- 175kd
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab02730(EIF5B antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human gliomas using FNab02730(EIF5B antibody) at dilution of 1:100
- Background
- Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
