Products
EIF4A2 antibody
- Product Name
- EIF4A2 antibody
- Catalogue No.
- FNab02717
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- eukaryotic translation initiation factor 4A, isoform 2
- Alternative Names
- BM 010 antibody, DDX2B antibody, eIF 4A II antibody, EIF4A antibody, eIF4A II antibody, EIF4A2 antibody, EIF4F antibody
- UniProt ID
- Q14240
- Observed MW
- 47kd
Application
- Tested Applications
- ELISA, WB, IF, IHC, IP
- Recommended dilution
- WB: 1:200-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab02717(EIF4A2 antibody) at dilution of 1:400
IP Result of anti-EIF4A2 (IP:FNab02717, 4ug; Detection:FNab02717 1:500) with Jurkat cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human ovary tumor using FNab02717(EIF4A2 antibody) at dilution of 1:100
- Background
- ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
