Products
EIF2S1 antibody
- Product Name
- EIF2S1 antibody
- Catalogue No.
- FNab02699
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
- Alternative Names
- EIF2A antibody
- UniProt ID
- P05198
- Observed MW
- 36 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, FC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
human stomach tissue were subjected to SDS PAGE followed by western blot with FNab02699(EIF2S1 antibody) at dilution of 1:500
IP Result of anti-EIF2S1 (IP:FNab02699, 4ug; Detection:FNab02699 1:1000) with HepG2 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human colon cancer using FNab02699(EIF2S1 antibody) at dilution of 1:50
- Background
- Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
