Products

ECHS1 antibody

Catalogue No.:FNab02624Reativity:Human
Host:MouseTested Application:ELISA, WB, IHC, IP, IF
Clonality:monoclonalIsotype:IgG1
  • SPECIFICATIONS
Product Name
ECHS1 antibody
Catalogue No.
FNab02624
Size
100μg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG1
Clone ID
1H0
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
UniProt ID
P30084
Observed MW
31 kDa
Application
Tested Applications
ELISA, WB, IHC, IP, IF
Recommended dilution
WB: 1:2000-1:20000; IP: 1:500-1:2000; IHC: 1:50-1:1000; IF: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab02624(ECHS1 antibody) at dilution of 1:1000
IP Result of anti-ECHS1 (IP:FNab02624, 3ug; Detection:FNab02624 1:500) with PC-3 cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using FNab02624(ECHS1 Antibody) at dilution of 1:50
Background
Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.