ECHS1 antibody

Catalogue No.:FNab02623Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
ECHS1 antibody
Catalogue No.
FNab02623
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
UniProt ID
P30084
Observed MW
31 kDa
Application
Tested Applications
ELISA, WB, IHC, IP
Recommended dilution
WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
human liver tissue were subjected to SDS PAGE followed by western blot with FNab02623(ECHS1 antibody) at dilution of 1:1200
IP Result of anti-ECHS1 (IP:FNab02623, 3ug; Detection:FNab02623 1:500) with PC-3 cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human prostate cancer using FNab02623(ECHS1 antibody) at dilution of 1:100
Background
Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.