Products
DNMT1 antibody
- Product Name
- DNMT1 antibody
- Catalogue No.
- FNab02482
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- DNA(cytosine-5-)-methyltransferase 1
- Alternative Names
- AIM antibody, CXXC9 antibody, DNA MTase HsaI antibody, DNMT antibody, DNMT1 antibody, MCMT antibody
- UniProt ID
- P26358
- Observed MW
- 180-200 kDa
Application
- Tested Applications
- ELISA, IHC, IP, WB, IF
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
293T cells were subjected to SDS PAGE followed by western blot with FNab02482(DNMT1 antibody) at dilution of 1:1000
IP Result of anti-DNMT1 (IP:FNab02482, 5ug; Detection:FNab02482 1:500) with HEK-293 cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab02482(DNMT1 antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using FNab02482 (DNMT1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
- Background
- Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes(TSGs) or other tumor-related genes in colorectal cancer(CRC) cells(PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells(ESCs)(PubMed:24623306). Associates at promoter regions of tumor suppressor genes(TSGs) leading to their gene silencing(PubMed:24623306). Promotes tumor growth(PubMed:24623306).
