Products
DIABLO antibody
- Product Name
- DIABLO antibody
- Catalogue No.
- FNab02384
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- diablo homolog(Drosophila)
- Alternative Names
- DIABLO antibody, Diablo homolog antibody, mitochondrial antibody, DIABLO S antibody, SMAC antibody, SMAC3 antibody
- UniProt ID
- Q9NR28
- Observed MW
- 20 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab02384(DIABLO antibody) at dilution of 1:1000
IP Result of anti-DIABLO (IP:FNab02384, 4ug; Detection:FNab02384 1:500) with HEK-293 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human testis using FNab02384(DIABLO antibody) at dilution of 1:50
- Background
- Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins(IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.This antibody recognizes all the three isoforms of DIABLO.
