SKOV-3 cells were subjected to SDS PAGE followed by western blot with FNab02332( DENND1A Antibody) at dilution of 1:600
Background
Guanine nucleotide exchange factor(GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles and mediates exit from early endosomes(PubMed:20154091, PubMed:20937701). Binds phosphatidylinositol-phosphates(PtdInsPs), with some preference for PtdIns(3)P(By similarity).