Products
DCUN1D1 antibody
- Product Name
- DCUN1D1 antibody
- Catalogue No.
- FNab02278
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- DCN1, defective in cullin neddylation 1, domain containing 1
- Alternative Names
- DCUN1L1 antibody, RP42 antibody, SCCRO antibody
- UniProt ID
- Q96GG9
- Observed MW
- 28 kDa
Application
- Tested Applications
- ELISA, IHC, WB
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab02278(DCUN1D1 Antibody) at dilution of 1:3000
Immunohistochemistry of paraffin-embedded human gliomas using FNab02278(DCUN1D1 antibody) at dilution of 1:50
- Background
- Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8(UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates(PubMed:25349211). Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression(By similarity).
