Products
DACH1 antibody
- Product Name
- DACH1 antibody
- Catalogue No.
- FNab02230
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- dachshund homolog 1(Drosophila)
- Alternative Names
- DACH antibody
- UniProt ID
- Q9UI36
- Observed MW
- 97-100 kDa
Application
- Tested Applications
- ELISA, IHC, IP, WB
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab02230(DACH1 Antibody) at dilution of 1:600
IP Result of anti-DACH1 (IP:FNab02230, 4ug; Detection:FNab02230 1:1000) with HEK-293 cells lysate 6000ug.
Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using FNab02230(DACH1 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
- Background
- Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter(By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain(By similarity).
