Products
CREST antibody
- Product Name
- CREST antibody
- Catalogue No.
- FNab01972
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 7A7
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- synovial sarcoma translocation gene on chromosome 18-like 1
- Alternative Names
- CREST antibody, KIAA0693 antibody, LP2261 antibody, SS18 like protein 1 antibody, SS18L1 antibody, SYT homolog 1 antibody
- UniProt ID
- O75177
- Observed MW
- 50 kDa
Application
- Tested Applications
- ELISA, WB
- Recommended dilution
- WB: 1:1000-1:6000
Validated Images
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab01972(CREST antibody) at dilution of 1:3000
- Background
- Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein(CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP(By similarity).
