Products
COPE antibody
- Product Name
- COPE antibody
- Catalogue No.
- FNab01865
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- coatomer protein complex, subunit epsilon
- UniProt ID
- O14579
- Observed MW
- 36 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
PC-3 cells were subjected to SDS PAGE followed by western blot with FNab01865(COPE antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using FNab01865(COPE Antibody) at dilution of 1:50
- Background
- The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors(ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors(By similarity).
