HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab01796(CNGA3 antibody) at dilution of 1:600
Background
Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3(By similarity). Essential for the generation of light-evoked electrical responses in the red-, green-and blue sensitive cones.