K-562 cells were subjected to SDS PAGE followed by western blot with FNab01692(CHST12 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using FNab01692(CHST12 antibody) at dilution of 1:50
Background
Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine(GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.