CHEK1 antibody

Synonyms:Serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Cell cycle checkpoint kinase|Checkpoint kinase-1|CHEK1|CHK1 antibody
Catalogue No.:FNab01653Reactivity:Human, Mouse, Rat
Host:MouseTested Application:ELISA, WB, IHC
Clonality:monoclonalIsotype:IgG2a
  • SPECIFICATIONS
Product Name
CHEK1 antibody
Catalogue No.
FNab01653
Size
100μg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG2a
Clone ID
9F10
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
CHK1 checkpoint homolog
Alternative Names
Serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Cell cycle checkpoint kinase|Checkpoint kinase-1|CHEK1|CHK1 antibody
UniProt ID
O14757
Observed MW
50 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab01653(CHK1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab01653(CHK1 Antibody) at dilution of 1:50
Background
HEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.