Products
CHAF1A antibody
- Product Name
- CHAF1A antibody
- Catalogue No.
- FNab01630
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- chromatin assembly factor 1, subunit A(p150)
- Alternative Names
- CAF antibody, CAF1P150 antibody
- UniProt ID
- Q13111
- Observed MW
- 150 kDa
Application
- Tested Applications
- ELISA, IHC, WB, IP, IF
- Recommended dilution
- WB: 1:1000-1:4000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01630(CHAF1A antibody) at dilution of 1:2000
IP Result of anti-CHAF1A (IP:FNab01630, 4ug; Detection:FNab01630 1:1000) with Jurkat cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human cervix tissue slide using FNab01630(CHAF1A Antibody) at dilution of 1:50
- Background
- Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci(By similarity).
