Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01522(CDC25B antibody) at dilution of 1:300
IP Result of anti-CDC25B (IP:FNab01522, 4ug; Detection:FNab01522 1:300) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human lymphoma using FNab01522(CDC25B antibody) at dilution of 1:100
Background
CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).