Products

CDC25B antibody

Synonyms:CDC25B antibody, CDC25HU2 antibody, M phase inducer phosphatase 2 antibody
Catalogue No.:FNab01522Reativity:Human, Mouse
Host:RabbitTested Application:ELISA, WB, IHC, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
CDC25B antibody
Catalogue No.
FNab01522
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
cell division cycle 25 homolog B(S. pombe)
Alternative Names
CDC25B antibody, CDC25HU2 antibody, M phase inducer phosphatase 2 antibody
UniProt ID
P30305
Observed MW
60-70 kDa
Application
Tested Applications
ELISA, WB, IHC, IP
Recommended dilution
WB: 1:200-1:1000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01522(CDC25B antibody) at dilution of 1:300
IP Result of anti-CDC25B (IP:FNab01522, 4ug; Detection:FNab01522 1:300) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human lymphoma using FNab01522(CDC25B antibody) at dilution of 1:100
Background
CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).