Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01518( CDC20 Antibody) at dilution of 1:800
IP Result of anti-CDC20 (IP:FNab01518, 4ug; Detection:FNab01518 1:800) with Jurkat cells lysate 3200ug.
Background
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome(APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.