Caspase 9 antibody

Synonyms:APAF 3 antibody, APAF3 antibody, Apoptotic protease Mch 6 antibody, CASP 9 antibody, CASP9 antibody, Caspase 9 antibody, CASPASE 9c antibody, Caspase9 antibody, Caspase-9 antibody, ICE LAP6 antibody, ICE like apoptotic protease 6 antibody, MCH6 antibody
Catalogue No.:FNab01295Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, IF, IHC, IP, WB, FC
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
Caspase 9 antibody
Catalogue No.
FNab01295
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
caspase 9, apoptosis-related cysteine peptidase
Alternative Names
APAF 3 antibody, APAF3 antibody, Apoptotic protease Mch 6 antibody, CASP 9 antibody, CASP9 antibody, Caspase 9 antibody, CASPASE 9c antibody, Caspase9 antibody, Caspase-9 antibody, ICE LAP6 antibody, ICE like apoptotic protease 6 antibody, MCH6 antibody
UniProt ID
P55211
Observed MW
46kd
Application
Tested Applications
ELISA, IF, IHC, IP, WB, FC
Recommended dilution
WB: 1:200-1:1000; IP: 1:200-1:1000; IHC: 1:50-1:200; IF: 1:10-1:100
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab01295(Caspase 9 Antibody) at dilution of 1:1000
IP result of anti-Caspase 9(IP:FNab01295, 3ug; Detection:FNab01295 1:200) with HeLa cells lysate 2500 ug
Immunohistochemistry of paraffin-embedded mouse lung using FNab01295(CASP9 antibody) at dilution of 1:50
Background
Caspase 9, apoptosis-related cysteine protease(CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. FNab01295 can recognize the pre-and cleaved-caspase 9.