Products
Caspase 8 antibody
- Product Name
- Caspase 8 antibody
- Catalogue No.
- FNab01293
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- caspase 8, apoptosis-related cysteine peptidase
- Alternative Names
- Apoptotic cysteine protease antibody, Apoptotic protease Mch 5 antibody, Casp 8 antibody, CASP8 antibody, Caspase 8 antibody, Caspase 8/p18 antibody, Caspase8 antibody, FADD like ICE antibody, FLICE antibody, ICE like apoptotic protease 5 antibody, MACH antibody, MCH5 antibody
- UniProt ID
- Q14790
- Observed MW
- 50-55 kDa, 25-32 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP, IF
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:100; IF: 1:10-1:100
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab01293(CASP8 antibody) at dilution of 1:1000
IP Result of anti-CASP8 (IP:FNab01293, 3ug; Detection:FNab01293 1:500) with HeLa cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human lymphoma using FNab01293(CASP8 antibody) at dilution of 1:50
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using FNab01293 （Caspase 8 Antibody）at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
- Background
- Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816). It may control diabetic embryopathy-associated apoptosis via Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibodycan recognize pro-and cleaved-caspase 8.
