Calbindin antibody

Synonyms:CAB27 antibody, CALB antibody, CALB1 antibody, Calbindin antibody, calbindin 1 antibody, 28kDa antibody, Calbindin D28 antibody, D 28K antibody
Catalogue No.:FNab01193Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB,IHC,IP,IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
Calbindin antibody
Catalogue No.
FNab01193
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
calbindin 1, 28kDa
Alternative Names
CAB27 antibody, CALB antibody, CALB1 antibody, Calbindin antibody, calbindin 1 antibody, 28kDa antibody, Calbindin D28 antibody, D 28K antibody
UniProt ID
P05937
Observed MW
30kd
Application
Tested Applications
ELISA, WB,IHC,IP,IF
Recommended dilution
WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500
Validated Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab01193(Calbindin-D28k antibody) at dilution of 1:1000
IP Result of anti-Calbindin-D28k (IP:FNab01193, 4ug; Detection:FNab01193 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab01193(Calbindin-D28k antibody at dilution of 1:100
Immunofluorescent analysis of ( 4% PFA ) fixed mouse cerebellum tissue using FNab01193 (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.