mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab01193(Calbindin-D28k antibody) at dilution of 1:1000
IP Result of anti-Calbindin-D28k (IP:FNab01193, 4ug; Detection:FNab01193 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab01193(Calbindin-D28k antibody at dilution of 1:100
Immunofluorescent analysis of ( 4% PFA ) fixed mouse cerebellum tissue using FNab01193 (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.