Products
C5AR1 antibody
- Product Name
- C5AR1 antibody
- Catalogue No.
- FNab01118
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- complement component 5a receptor 1
- Alternative Names
- C5AR antibody, C5R1 antibody
- UniProt ID
- P21730
- Observed MW
- 45-50 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
PC-3 cells were subjected to SDS PAGE followed by western blot with FNab01118(C5AR1 antibody) at dilution of 1:800
IP Result of anti-C5AR1 (IP:FNab01118, 4ug; Detection:FNab01118 1:600) with mouse liver tissue lysate 6400ug.
Immunohistochemistry of paraffin-embedded human liver using FNab01118(C5AR1 antibody) at dilution of 1:50
- Background
- Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a(PubMed:1847994, PubMed:8182049, PubMed:7622471, PubMed:9553099, PubMed:10636859, PubMed:15153520). The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events(PubMed:8182049, PubMed:7622471, PubMed:9553099). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production(PubMed:10636859, PubMed:15153520).
