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BORIS antibody
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Synonyms: | BORIS antibody, Cancer/testis antigen 27 antibody, CCCTC binding factor antibody, CT27 antibody, CTCF like protein antibody, CTCF paralog antibody, CTCF T antibody, CTCFL antibody, dJ579F20.2 antibody, Zinc finger protein CTCF T antibody | ||
Catalogue No.: | FNab00932 | Reativity: | Human, Mouse |
Host: | Mouse | Tested Application: | ELISA, WB, IF |
Clonality: | monoclonal | Isotype: | IgG1 |
- SPECIFICATIONS
- Product Name
- BORIS antibody
- Catalogue No.
- FNab00932
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 2B10
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- CCCTC-binding factor(zinc finger protein)-like
- Alternative Names
- BORIS antibody, Cancer/testis antigen 27 antibody, CCCTC binding factor antibody, CT27 antibody, CTCF like protein antibody, CTCF paralog antibody, CTCF T antibody, CTCFL antibody, dJ579F20.2 antibody, Zinc finger protein CTCF T antibody
- UniProt ID
- Q8NI51
- Observed MW
- 75 kDa
Application
- Tested Applications
- ELISA, WB, IF
- Recommended dilution
- WB: 1:500-1:2000; IF: 1:10-1:100
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab00932(BORIS antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells, using FNab00932(BORIS antibody) at 1:25 dilution andRhodamine-labeled goat anti-mouse IgG (red).
- Background
- CTCFL, also named BORIS(for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region(ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.