BCKDHB antibody

Catalogue No.:FNab00834Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
BCKDHB antibody
Catalogue No.
FNab00834
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
branched chain keto acid dehydrogenase E1, beta polypeptide
UniProt ID
P21953
Observed MW
43 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00834(BCKDHB Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using FNab00834(BCKDHB Antibody) at dilution of 1:50
Background
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase(E1), lipoamide acyltransferase(E2) and lipoamide dehydrogenase(E3).