Products
BBS5 antibody
- Product Name
- BBS5 antibody
- Catalogue No.
- FNab00819
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Bardet-Biedl syndrome 5
- UniProt ID
- Q8N3I7
- Observed MW
- 40-45 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC, IP
- Recommended dilution
- WB: 1:1000-1:6000; IP: 1:500-1:3000; IHC: 1:50-1:500; IF: 1:50-1:500
Validated Images
mouse testis tissue were subjected to SDS PAGE followed by western blot with FNab00819(BBS5 antibody) at dilution of 1:400
IP result of anti-BBS5(IP:FNab00819, 4ug; Detection:FNab00819 1:600) with mouse testis tissue lysate 4000 ug
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab00819(BBS5 Antibody) at dilution of 1:200
- Background
- The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor(GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog(SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.
