Products
AXL antibody
- Product Name
- AXL antibody
- Catalogue No.
- FNab00754
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- AXL receptor tyrosine kinase
- Alternative Names
- AXL antibody, AXL oncogene antibody, AXL receptor tyrosine kinase antibody, JTK11 antibody, UFO antibody
- UniProt ID
- P30530
- Observed MW
- 140 kDa
Application
- Tested Applications
- ELISA, WB, IP, FC
- Recommended dilution
- WB: 1:500-1:3000; IP: 1:500-1:2000
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00754(AXL antibody) at dilution of 1:500
IP Result of anti-AXL (IP:FNab00754, 5ug; Detection:FNab00754 1:1000) with HepG2 cells lysate 9000ug.
- Background
- Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors(TLRs)-mediated innate immune response. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope(PubMed:22156524, PubMed:22673088, PubMed:25277499, PubMed:21501828). Acts as a receptor for ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope(PubMed:17005688).
